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Anti-Puromycin [3RH11] Antibody, 100ug
Anti-Puromycin [3RH11] Antibody, 100ug from Kerafast
(1)
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Citations:
(145)
Supplier Page
Supplier Page from
Kerafast for
Anti-Puromycin [3RH11] Antibody, 100ug
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Kerafast's
Anti-Puromycin [3RH11] Antibody, 100ug
is a Mouse monoclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, Microscopy, ELISA, Immunofluorescence, and Western Blot.
Product Specs
Company Info
Citations
Reviews
Bench Tips
Product Specs
Item
Anti-Puromycin [3RH11] Antibody, 100ug
Company
Kerafast
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
EQ0001
Quantity
100ug
Applications
Western blotting, ELISA and Immunofluorescence microscopy
Format
Liquid
Purity
Protein G
Type
Antibody
Target
Puromycin
Clone
3RH11
Immunogen
puromycin hydrochloride
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Add to Compare List
Kerafast
2 Shaker Road, Unit B101
Shirley
,
MA
01464
United States
Phone
: 1-800-546-1760
1-617-336-3228 (Mon–Fri 8:30AM–5:30PM EST)
Fax: 1-866-727-9826
Email: CustomerService@kerafast.com
Company Profile
Website:
www.kerafast.com
Citations (145)
(1)
Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processing
RNA Biology
December 31, 2023
Mason A. McCool, Carson J. Bryant, Hannah Huang, Lisa M. Ogawa, Katherine I. Farley-Barnes, Samuel B. Sondalle, Laura Abriola, Yulia V. Surovtseva, Susan J. Baserga
blotting
. The following primary antibodies were used: α-
p53
(
Santa Cruz
,
sc-126
), α-puromycin (
Kerafast
,
EQ0001
), α-HA (
clone
12CA5), and α-β-actin (
Sigma Aldrich
,
A1978
), α-POLR1A (
Santa Cruz
,
sc-48
,385), α-
FBL
(2)
microRNAs slow translating ribosomes to prevent protein misfolding in eukaryotes
The EMBO Journal
September 18, 2023
Hiroaki Sako, Mohieldin Youssef, Olga Elisseeva, Takayuki Akimoto, Katsuhiko Suzuki, Takashi Ushida, Tadashi Yamamoto
Millipore
), anti‐
AGO2
(
2897S
,
Cell Signaling
), anti‐
DROSHA
(
sc‐393591
,
Santa Cruz
), anti‐puromycin (
EQ0001
,
Kerafast
), anti‐
TUBG1
(
T3320
,
Sigma
), anti‐
GAPDH
(
2118S
,
Cell Signaling
), anti‐rabbit IgG (NA9340,
(3)
AGAP1-associated endolysosomal trafficking abnormalities link gene–environment interactions in neurodevelopmental disorders
Disease Models & Mechanisms
September 1, 2023
Sara A. Lewis, Somayeh Bakhtiari, Jacob Forstrom, Allan Bayat, Frédéric Bilan, Gwenaël Le Guyader, Ebba Alkhunaizi, Hilary Vernon, Sergio R. Padilla-Lopez, Michael C. Kruer
(
1:500
; Abcam 4837). Newly synthesized proteins were labeled with
mouse
anti-puromycin (
1:1000
;
Kerafast
,
EQ0001
) and their levels normalized using Ponceau
staining
. The following secondary antibodies were used:
goat
(4)
The translation initiating factor eIF4E and arginine methylation underlie G3BP1 function in dendritic spine development of neurons
The Journal of biological chemistry
August 1, 2023
Dong R, Li X, Flores AD, Lai KO.
BSA in D-PBS) for 30 min. After blocking, the cells were incubated with puromycin antibody (
Kerafast
#
EQ0001
,
1:500
diluted in blocking buffer) overnight at 4 °C and subjected to
immunofluorescence
staining
on
(5)
Sequence- and structure-specific RNA oligonucleotide binding attenuates heterogeneous nuclear ribonucleoprotein A1 dysfunction
Frontiers in Molecular Biosciences
June 22, 2023
Joseph P. Clarke, Patricia A. Thibault, Sakina Fatima, Hannah E. Salapa, Subha Kalyaanamoorthy, Aravindhan Ganesan, Michael C. Levin
anti-hnRNPA1 (4B10),
Millipore Sigma
,Cat#
05-1521
Mouse
monoclonal
anti-Puromycin [3RH11
],
Kerafast
,Cat#
EQ0001
Alexa Fluor 488 Donkey anti-mouse IgG (H+L),
Jackson ImmunoResearch
,Cat#
715-546-151
Alexa Fluor 488
(6)
NAD(+) repletion with niacin counteracts cancer cachexia
Nature communications
April 3, 2023
Beltrà M, Pöllänen N, Fornelli C, Tonttila K, Hsu MY, Zampieri S, Moletta L, Corrà S, Porporato PE, Kivelä R,
et al
.
(
1:1000
,
AB3242
,
Merck Millipore
, polyclonal), PINK1 (
1:500
,
SAB2500794
, polyclonal), Puromycin (
1:1000
,
EQ0001
,
Kerafast
,
clone
3RH11),
TOMM20
(
1:1000
,
ab186735
,
Abcam
,
clone
EPR15581-54) and Vinculin (
1:2000
,
sc73614
,
(7)
AGAP1-associated endolysosomal trafficking abnormalities link gene-environment interactions in a neurodevelopmental disorder
bioRxiv
February 4, 2023
Sara A. Lewis, Somayeh Bakhtiari, Jacob Forstrom, Allan Bayat, Frédéric Bilan, Gwenaël Le Guyader, Ebba Alkhunaizi, Hilary Vernon, Sergio R. Padilla-Lopez, Michael C. Kruer
α
eIF2S1
(Abcam 4837). Newly synthesized proteins labeled with
1:1000
mouse
α puromycin (
Kerafast
,
EQ0001
) and normalized to Ponceau
staining
. Secondary antibodies:
1:10000
goat
α rabbit or
goat
α
mouse
ECL
(8)
Pharmacological vitamin C inhibits mTOR signaling and tumor growth by degrading Rictor and inducing HMOX1 expression
PLoS genetics
February 1, 2023
Qin S, Wang G, Chen L, Geng H, Zheng Y, Xia C, Wu S, Yao J, Deng L.
Cell Signaling Technology
), p4EBP1 (
1:1000
;
9451S
;
Cell Signaling Technology
), anti-Puromycin (
1:1000
,
EQ0001
,
Kerafast
),
SVCT1
(
1:1000
;
NBP2-13318
;
Novus
), SVCT2 (
1:1000
;
NBP2-13319
;
Novus
),
AMPK
(
1:1000
;
2532S
;
(9)
Real-time imaging of RNA polymerase I activity in living human cells
The Journal of cell biology
January 2, 2023
Fu Y, Liu Y, Wen T, Fang J, Chen Y, Zhou Z, Gu X, Wu H, Sheng J, Xu Z,
et al
.
to polyvinylidene difluoride
membranes
followed by
incubation
with anti-puromycin antibody (
Kerafast
-
EQ0001
, 1 µg/ml in Tris-buffered saline containing Tween-20) overnight at 4°C, goat anti-mouse HRP secondary
(10)
Intensive Care Unit Acquired Weakness Is Associated with Rapid Changes to Skeletal Muscle Proteostasis
Cells
December 11, 2022
Mustafa Ozdemir, Matthew P. Bomkamp, Hayden W. Hyatt, Ashley J. Smuder, Scott K. Powers
software (Li-Cor Biosciences, Lincoln, NE, USA). Primary antibodies of interest were anti-puromycin (#
EQ0001
) (
Kerafast
, Boston, MA, USA), anti-
mTOR
(#
2983
), anti-p-mTOR (#
5536
), anti-
AKT
(#
2938
), anti-p-AKT (#
9271
),
(11)
Human pre-60S assembly factors link rRNA transcription to pre-rRNA processing
RNA (New York, N.Y.)
November 2, 2022
McCool MA, Buhagiar AF, Bryant CJ, Ogawa LM, Abriola L, Surovtseva YV, Baserga SJ.
Santa Cruz
sc-126
), α-
p21
(dilution
1:400
,
Santa Cruz
sc-6246
), α-puromycin (dilution
1:10,000
;
Kerafast
EQ0001
), and α-β-actin (dilution
1:30,000
;
Sigma Aldrich
A1978
). The
western blots
were developed with enhanced
(12)
Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells
Science advances
October 28, 2022
Datta C, Truesdell SS, Wu KQ, Bukhari SIA, Ngue H, Buchanan B, Le Tonqueze O, Lee S, Kollu S, Granovetter MA,
et al
.
(
ab134053
), and
ATF4
(
ab23760
) (used
for Western blot
) were from
Abcam
; and
anti-puromycin [3RH11
] (
EQ0001
) was from
Kerafast
.
Mass spectrometry
Multiplex
quantitative proteomics analysis (TMT
spectrometry
)
(13)
miR-124-dependent tagging of synapses by synaptopodin enables input-specific homeostatic plasticity
The EMBO journal
October 17, 2022
Dubes S, Soula A, Benquet S, Tessier B, Poujol C, Favereaux A, Thoumine O, Letellier M.
modifications (Merck).
Labeling
of newly synthesized SP was performed using
mouse
anti‐puromycin antibody (
EQ0001
,
Kerafast
,
1:2,500
) in combination with rabbit anti‐SP antibody (
163002
, Synaptic System,
1:600
) and
(14)
Therapeutic implications of mitochondrial stress-induced proteasome inhibitor resistance in multiple myeloma
Science advances
September 30, 2022
Sharma A, Nair R, Achreja A, Mittal A, Gupta P, Balakrishnan K, Edgar CL, Animasahun O, Dwivedi B, Barwick BG,
et al
.
were obtained from
Sigma-Aldrich
.
REDD1
(#
10638-1-AP
) was obtained from
Proteintech
. Anti-puromycin (#
EQ0001
) was obtained from
Kerafast
. Bioinformatics analysis Unpaired analysis GSEA was performed on DESeq2-normalized
(15)
Mettl3-dependent m
6
A modification attenuates the brain stress response in Drosophila
Nature communications
September 14, 2022
Perlegos AE, Shields EJ, Shen H, Liu KF, Bonini NM.
10/10/19–53 ug/ml), anti-drpr (
1:400
, DSHB-5D14, 6/22/17−36 ug/ul), anti-puromycin (
1:1,000
,
Kerafast
,
EQ0001
, 200517), anti-hsf (
1:20
:000, anti-rabbit, gift from John Lis60,61). Rabbit anti-Ythdc1 (
1:5000
), affinity
(16)
4-Hydroxyisoleucine inhibits tumor growth by triggering endoplasmic reticulum stress and autophagy
Current Research in Pharmacology and Drug Discovery
September 9, 2022
Peng Li, Yonghui Yang, Lu Qiu, Guangming Wan, Baomei Yuan, Yahong Wu, Yanfeng Gao, Guodong Li
PERK
(
Abcam
,
ab229912
,UK), p-PERK(Affinity, DF-7576),
LC3B
(
Abcam
,
ab192890
, UK),
Puromycin
(
Kerafast
,
EQ0001
,USA). The band density was measured (Quantity One software) and normalized to β-Actin. 2.7
Transmission electron microscopy
(17)
White spot syndrome virus directly activates mTORC1 signaling to facilitate its replication via polymeric immunoglobulin receptor-mediated infection in shrimp
PLOS Pathogens
September 6, 2022
Pan-Pan Hong, Cang Li, Guo-Juan Niu, Xiao-Fan Zhao, Jin-Xing Wang
(p)-S6K1 (Thr389) (Catalog No.
AP0564
,
ABclonal
, Wuhan, China,
1:500
); anti-puromycin (Catalog No.
EQ0001
,
Kerafast
,
1:1000
), and gently shaken overnight at 4°C. After washing three times with TBST (0.1% Tween-20
(18)
L-Arginine/nitric oxide regulates skeletal muscle development via muscle fibre-specific nitric oxide/mTOR pathway in chickens
Animal Nutrition
September 1, 2022
Ruxia Wang, Kelin Li, Li Sun, Hongchao Jiao, Yunlei Zhou, Haifang Li, Xiaojuan Wang, Jingpeng Zhao, Hai Lin
Boston, USA), anti-p70S6K (#
2708
, anti-rabbit,
Cell Signaling Technology
, Boston, USA), anti-puromycin (
EQ0001
, anti-
mouse
,
Kerafast
, Boston, USA), and anti-tubulin (AT819, anti-
mouse
, Beyotime, Nanjing, China)
(19)
Serotonergic neuron ribosomal proteins regulate the neuroendocrine control of Drosophila development
PLOS Genetics
September 1, 2022
Lisa Patricia Deliu, Michael Turingan, Deeshpaul Jadir, Byoungchun Lee, Abhishek Ghosh, Savraj Singh Grewal
Primary antibodies used were
anti-puromycin (3RH11
) antibody (
1:1000
,
Kerafast
, Boston, USA, cat. no.
EQ0001
), anti-eIF2alpha (
1:1000
, AbCam #26197). Secondary antibodies were purchased from
Santa Cruz
144 Biotechnology
(20)
An aqueous extract of the brown alga Eisenia bicyclis extends lifespan in a sex-specific manner by interfering with the Tor-FoxO axis
Aging (Albany NY)
August 31, 2022
Navid Tahanzadeh, Mirjam Knop, Yvonne Seidler, Sebastian Dirndorfer, Kai Lürsen, Iris Bruchhaus, Roman Lang, Gerald Rimbach, Thomas Roeder
for 1.30 h. The
membrane
was incubated with
anti-puromycin (3RH11
) antibody (
Kerafast
, Boston, USA,
EQ0001
) over night at 4° C. After 3 times washing with TBST, HRP-coupled anti-rabbit secondary antibody was
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Reviews
Write a Review
(1)
6/13/2022
Good Puromycin Antibody for Western Blot
Chang Seok Lee
A mouse model with I4895T mutation in type 1 ryanodine receptor displays myopathy including muscle weakness and atrophy. In vivo protein synthesis with puromycin labeling showed decrease of newly ...
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Bench Tips
Bench Tips for Optimizing ELISA: It All Comes Out in the Wash
August 22, 2013
This article will provide you with tips on washing your ELISA plates using an automated plate washer, with the goal of avoiding high background and high variation (between plates and between replicate wells).
read more
Anti-Puromycin [3RH11] Antibody, 100ug from Kerafast
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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